FMR | Fumarate reductase
AS12 2618 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii
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1 : 1000 with alkaline phosphatase (WB)
|Expected | apparent MW||
|Predicted reactivity||Leishmania mexicana, Naegleria gruberi (Amoeba)|
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
to be added when available
Subramanian et al. (2014). Profiling Chlamydomonas Metabolism under Dark, Anoxic H 2 ‐Producing Conditions Using a Combined Proteomic, Transcriptomic, and Metabolomic Approach. J Proteome Res. 2014 Oct 21.
25 µg of total protein from Chlamydomonas reinhardtii, oxic conditions (A), dark anoxia (B) were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF. Blotting was done using SNAP-ID kit: incubation in blocking buffer for 1 min., following incubation in a primary antibody at a dilution of 1: 1 000 for 20 min, wash three times with wash buffer TBS-T, followed by incubation ina secondary antibody at a dilution of 1: 5000, for 20 min. and three times wash in TBS-T. The blot was washed and developed with AP color development reagent (AP conjugate substrate kit from Bio-rad) according to the manufacturer's instructions.
Courtesy of Dr. Alexandra Dubini
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