FtsZ | Procaryotic cell division GTPase
AS10 715 | clonality: polyclonal | host: rabbit | reactivity: E.coli DH5a, Shigella flexneri
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1 : 1000 with standard ECL (WB), 1:100 - 1: 200 (IF)
|Expected | apparent MW||
40 | 42 kDa
|Confirmed reactivity||E coli DH5a, Shigella flexneri|
Candidatus sp., Citrobacter sp. 30_2, Dickeya sp., Enterobacter sp., Klebsiella pneumoniae subsp. pneumoniae MGH, Salmonella sp., Shigella sonnei Ss046, Vibrio sp., Yersinia pestis D182038
|Not reactive in||
B. subtilis, Listera sp., Neisseria meningitidis, Pseudomonas aeruginosa, cyanobacteria
to be added when available
|Selected references||Pende et al. (2014). Size-independent symmetric division in extraordinarily long cells. Nat Commun. 2014 Sep 15;5:4803. doi: 10.1038/ncomms5803.
Söderström et al. (2014). Disassembly of the divisome in Escherichia coli: Evidence that FtsZ dissociates before compartmentalisation. Mol Microbiol. 2014 Feb 7. doi: 10.1111/mmi.12534. (western blot and immunofluorescence)
5 µg of total protein from Synechocystis sp. (1), E.coli DH5a (2), E. coli (3), extracted with Agrisera PEB extraction buffer were separated on 4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked with Advance blocking reagent for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 120 seconds.
Total protein extract from E. coli CFT073: 8µg (1), 12µg (2), 16µg (3). Proteins were separated on 10% SDS-PAGE and blotted to PVDF. Blocked with 5 % non-fat milk in TBS-T for 1 hour. Blot was incubated in the primary antibody at a dilution of 1 :10 000 for 1 h at RT with agitation. Secondary antibody (anti-rabbit IgG, HRP conjugated, Agrisera, AS09 602) were diluted to 1 : 50 000 and blot was incubated for 1h at RT with agitation. Immunodetection was performed using ECL method for 3 min. Scan was made after 30 sec.
Courtesy of Dr. Marta Kicia, Wroclaw Medical University, Poland
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