FtsZ | procaryotic cell division GTPase

345 €

AS10 715 | clonality: polyclonal | host: rabbit | reactivity: E.coli DH5a, Shigella flexneri

PRODUCT INFORMATION IN PDF

product information

background

FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules. Synonymes: sifB, SulB.

immunogen

KLH-conjugated synthetic peptide derived from known bacterial sequences of FtsZ including E.coli P0A9A6

antibody format

rabbit

polyclonal

serum

lyophilized

quantity

200 µl

for reconstitution add 200 µl, of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB), immunofluorescence (IF)

related products

additional information

to be added when available

application information
recommended dilution		1 : 1000 with standard ECL (WB), 1: 200 (IF)
expected \| apparent MW		40 \| 42 kDa
confirmed reactivity		E coli DH5a, Shigella flexneri
predicted reactivity		Candidatus sp., Citrobacter sp. 30_2, Dickeya sp., Enterobacter sp., Klebsiella pneumoniae subsp. pneumoniae MGH, Salmonella sp., Shigella sonnei Ss046, Vibrio sp., Yersinia pestis D182038
not reactive in		B. subtilis, Listera sp., Pseudomonas aeruginosa, cyanobacteria
additional information		to be added when available
selected references		Fujihara et al. (2013). Effects of urea on length distribution and morphology of Escherichia coli and Salmonella enterica subsp. enterica cells. African Journal of Microb. Res. April 2013.

application example

western blot detection of bacterial FtsZ protein using anti-FtsZ antibody

5 µg of total protein from Synechocystis sp. (1), E.coli DH5a (2), E. coli (3), extracted with Agrisera PEB extraction buffer were separated on 4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked with Advance blocking reagent for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 120 seconds.

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