FtsZ2 | plant cell division protein ftsZ2

265 €
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AS13 2651| clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


26 st
Item No:
AS13 2651

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product information

FtsZ2 belongs to FtsZ family and binds to FtsZ1 and exhibits  GTPase activity. Component of a plastid division machinery.Alternative names: Plastid division protein FTSZ2-1, AtFtsZ2-1, Plastid division protein FTSZ2-2, AtFtsZ2-2.


Recombinant part of Arabidopsis thalina FtsZ2 conserved in FtsZ2-1 O82533
At2g36250, FtsZ2-2 Q9LXJ0, AT3G52750

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications western blot (WB)
Related products

AS07 217 | FtsZ | procaryotic cell division GTPase, cyanobacterial, rabbit antibody
AS09 413 | anti-FtsZ1 and 2 | plant cell division protein ftsZ1 and ftsZ2 , rabbit antibody
AS10 715 | anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information antibody available in 3 various pack sizes: 50, 100 and 150 µl - please, inquire.
application information
Recommended dilution 1: 2000 with enhanced ECL (WB)
Expected | apparent MW

50.7 kDa

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare

Predicted reactivity Glycine max, Jatropha manihot, Ricinus communis
Not reactive in no confirmed exceptions from predicted reactivity known in the moment
Additional information to be added when available
Selected references to be added when available, antibody released in June 2013.

application example

2 µg of recombinant FtsZ2 fragment(~10 kDa) (1) and total protein extracted with PEB (AS08 300) from leaf tissue Arabidopsis thaliana (2) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with FtsZ2 antibody (AS13 2651, 1:2000, 1h) and secondary anti-rabbit (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. The signal was developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 2 min.

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