GAPC1/2 | Glyceraldehyde-3-phosphate dehydrogenase (cytosolic)

Product no: AS15 2894

AS15 2894 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
319
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana GAPC1 and GAPC2 proteinsUniProt: P25858, Q9FX54

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 37 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Synechocystis sp. PCC 6803
    Predicted reactivity: Anthurium amnicola, Andrographis paniculata, Arachis ipaensis, Beta vulgaris, Brassica napus, Brassica olerace, Cajanus caja, Camelina sativa, Capsella rubella, Capsicum annuum, Carthamus tinctorius, Chlamydomonas reinhardtii, Cucumis sativus, Daucus carota, Elettaria cardamomum, Eleutherococcus senticosus, Eucalyptus grandis, Glycine max, Gymnadenia conopsea, Hordeum vulgare, Jatropha curcas, Mangifera indica, Malus domestica, Manihot esculenta, Medicago truncatula, Mikania micrantha, Nicotiana benthamiana, Oryza sativa, Phaseolus vulgaris, Prunus persica, Raphanus sativus, Rosmarinus officinalis, Salvia officinalis, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Tamarix hispida, Tarenaya hassleriana, Theobroma cacao, Triticum monococcum, Triticum aestivum, Vaccinium uliginosum, Vigna radiata, Vitis vinifera, Zostera marina
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application examples


    Western blot using anti-GAPC1/2 antibodies on cyanobacterial samples

    Samples:
    1 – Synechocystis sp. PCC 6803 wild type, 10 µg total protein extract
    2 – Synechocystis cp12 mutant, 10 µg total protein extract
    3 – Synechocystis gap1 mutant, 10 µg total protein extract
    4 – Arabidopsis thaliana wildtype, 10 µg whole leaf extract

    10 µg of total protein extracted freshly from Synechocystis sp. PCC 6803 with 1x PBS. Buffer components: 137 mM NaCl, 10 mM Na2HPO4*2H2O, 2 mM KH2PO4, 2,7 mM KCl, pH 7.4. 10 µg of whole leaf extract extracted from Arabidopsis thaliana. Extraction buffer components: 50 mM HEPES, 10 mM NaCl, 5mM MgCL2*6H2O, 100 mM Sorbitol, pH 7.6. Denatured at 95°C for 5 minutes with 3x Laemmli buffer, components: 150mM Tris-HCl (pH 6.8), 300 mM DTT, 6% SDS, 0.3% bromophenol blue, 30% glycerol. Samples were separated on 12% SDS-PAGE and blotted 90 minutes to PVDF membrane, using semi-dry transfer. Blot was blocked with 5% milk in 1xTBS for 1h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 with agitation in 1xTBS overnight at 4°C. The antibody solution was decanted, and the blot was rinsed twice, then washed 4 times for 10 minutes in 1xTBS at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 5% milk in 1xTBS for 2h/RT with agitation. The blot was washed as above and developed with AgriseraECLBright chemiluminescent solution. Exposure time was 10 seconds.

    Courtesy of Dr. Stefan Lucius, Universität Rostock, Germany


    Western blot using anti-GAPC1/2 antibodyMutants used for application example
    40 μg total proteins from Arabidopsis wt, and GAPC1-6xFlag, gapc1-1, gapac1-1/gapc2-1, and gapc1-1/gapc2-2 mutants, extracted and denatured as described by Larkin (2007), were separated on  12% SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with TBS-T buffer with 5% skimmed milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for overnight at 4°C  with agitation in TBS-T with 2% skimmed milk. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (Goat anti-rabbit IgG horse radish peroxidase conjugated, from ZSGB-BIO, China) diluted to 1:0000 in  for 1h at RT with agitation. The blot was washed as above and developed for 1 min with EasySee Western Blot Kit (TRANSTM, China). Exposure time was  30 seconds.

    Courtesy of Dr. Songhu Wang, Chengdu Institute of Biology, Chinese Academy of Sciences, China


    western blot using anti-GAPC1/2 antibodies on Arabidopsis

    50 µg of total protein from Arabidopsis thaliana extracted with buffer containing 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10 mM MgCl2, 5 mM EDTA, 10% glycerol, 0.1% NP-40, 0.1% protease inhibitor cocktail, and denatured by boiling for 5 min. The total proteins were separated on 10% SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10000 for 12 h at 4 0C in TBST containing 5% non-fat dry milk. The antibody solution was decanted and the blot was rinsed three times for 10 min each in TBST with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase or alkaline phosphatase conjugated) diluted to 1:10 000 in for 2 h at RT with agitation. The blot was washed as above and developed for 2 min with chemiluminescent detection reagent in extreme low femtogram range. Exposure time was 30 seconds. For alkaline phosphatase, the blot was stained with buffer (100 mM Trish pH9.5; 100 mM NaCl; 5 mM MgCl2) containing BCIP ((GoldBio) and NBT (Fischer Scientific).

    Courtesy of Dr. Pradeep Kachroo, University of Kentucky, USA
  • Additional Information
  • Additional information (application): Use of this antibody as a loading control should be supported with specific experimental data
  • Background
  • Background:

    Glyceraldehyde-3-phosphate dehydrogenase is an enzyme that catalyzes the first step in glycolysis by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. This enzyme is essential for the carbohydrate metabolism and the maintenance of cellular ATP levels. 

    Synonyme gene names: GAPC, GAPDH.

  • Product Citations
  • Selected references: Lee et al. (2023).Three consecutive cytosolic glycolysis enzymes modulate autophagic flux. Plant Physiol. 2023 Oct 26;193(3):1797-1815. doi: 10.1093/plphys/kiad439.
    Zhu et al. (2020). The RALF1-FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar Root Hair Growth. Mol Plant. 2020 May 4;13(5):698-716. doi: 10.1016/j.molp.2019.12.014..
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart
Info

AS13 2640 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stoloniferacv. ‘Penncross’,Arabidopsis thaliana, Brassica sp., Cannabis sativa L., Cucumis sativus, Cynara cardunculus, Glycine max, Hordeum vulgare, Nicotiana tabacum, Phaseolus vulgaris, Phoenix dactylifera, Picrorhiza kurroa, Setaria italica, Solanum tuberosum, Triticum aestivum, Zea mays

319 €
Add to cart
Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
Close