GFP | Green Fluorescence Protein (protein A purified)
AS15 3000 | Clonality: Polyclonal | Host: Rabbit | Reactivity: native and recombinant GFP
- Product Info
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Immunogen: highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
Host: Rabbit Clonality: Polyclonal Purity: Total IgG. Protein A purified. Format: Liquid Quantity: 100 µg at 1 mg/ml Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1 : 5000-1 : 25 000 (ELISA), 1 : 2000-1 : 10 000 (WB) - Reactivity
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Confirmed reactivity: Native GFP, Recombinant GFP (E.coli), all variants of GFP and EGFP - Application Examples
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Application example
EGFP-tagged nuclear lamins (in green), detected in the same cell by anti-GFP | Green Fluorescence Protein antibodies (in red; dilution 1:500).
Mouse fibroblasts were transfected with an EGFP-Lamin A hybrid construct and indirectly immunostained using anti-GFP | Green Fluorescence Protein as the primary antibody. Fixation and permeabilization was performed with 4% buffered formaldehyde for 10 min. at room temperature and treatment with 0.1% Triton X-100 for 10 min., respectively. - Additional Information
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Additional information (application): Minimal cross-reactivity with E.coli proteins
- Background
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Background: GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
- Product Citations
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Selected references: Wieczorek et al. (2020) Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants. Viruses. 2020 Oct 20;12(10):1195. doi: 10.3390/v12101195. PMID: 33092281; PMCID: PMC7588970.
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)