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GFP | Green Fluorescence Protein (total IgG)

281 €

AS15 2999 | clonality: polyclonal | host: rabbit | reactivity: native and recombinant GFP

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AS15 2999

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product information
Background

GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

Immunogen

highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG in 10 mM TRIS, pH 7.2 with 0.02 % sodium azide
Format
Quantity 100 ĩg
Reconstitution
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB)
Related products

AS15 2987 | GFP (affinity purified), rabbit antibody

AS15 3000 | GFP (protein A purified), rabbit antibody

AS15 3001 | GFP, peroxidase conjugated, rabbit antibody

AS15 2998 | GFP (total IgY), chicken antibody


AS15 3002 | GFP (purified recombinant protein)


other tag antibodies

Additional information
application information
Recommended dilution

1: 5000 - 1: 25 000 (ELISA), 1: 2000 - 1: 10 000 (WB)

Expected | apparent MW
Confirmed reactivity

native GFP, recombinant GFP (E.coli), all variants of GFP

Predicted reactivity
Not reactive in
Additional information

Minimal cross-reactivity with E.coli proteins

Selected references

to be added when available, antibody released in September 2015



||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at support@agrisera.com