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GSH (Glutathione) ELISA kit

545 €

AS16 4095 | GSH (Glutathione) ELISA kit

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Background | This ELISA kit uses Competitive-ELISA as the method. The provided microtiter plate is pre-coated with GSH.

During the reaction, GSH in the sample or standard competes with a fixed amount of GSH on the solid phase supporter for sites on the anti-
GSH biotinylated detection antibody. Excess conjugate and unbound sample or standard are washed away from the plate, and a secondary HRP-streptavidin (SABC) antibody is added to each microplate well and incubated. To develope the enzyme-substrate reaction a TMB substrate solution is added to each well and addition of a sulphuric acid solution terminates this reaction. Color change is measured spectrophotometrically at 450 nm wavelength. The concentration of GSH in the samples can be determined from the standard curve.


This kit contains following reagents:
Dismountable Micro ELISA Plate | 8×6 or 8×12 (4°C/-20°C)
Lyophilized Standard | 1 vial or 2 vial (4°C/-20°C)
Sample / Standard dilution buffer | 10 ml/20 ml
Biotin- detection antibody (Concentrated) | 30 μl/60 μl
Antibody dilution buffer | 5 ml/10 ml
HRP-Streptavidin Conjugate (SABC) | 60 μl/120 μl (4°C (dark storage)
SABC dilution buffer | 5 ml/10ml
TMB substrate | 5 ml/10 ml 4°C (dark storgae)
Stop solution | 5 ml/10 ml
Wash buffer (25X) | 15 ml/30 ml
Plate Sealer | 3/5 pieces


Size: 96 wells (allows to process 39 samples and a standard curve)
Assay range | 1.563-100 μg/ml 
Sensitivity | <0.55 μg/ml
Storage |  4°C for 6 months
Reactivity: Universal
Application:
For quantitative detection of GSH in a variety of samples.
WARNING: This kit is not for human or animal disease diagnosis or therapeutic drug use.



Precautions:

1. Before running your experimet we reccomend performing a pilot experiment using standards and a small amount of samples to establih the validity of experiment and dilutions.
2. Keep plate dry a
fter opening and before using.
3.
Before using this kit, spin tubes and bring down all components to the bottom of tubes.
4. Keep
TMB reagents in dark.
5. To prevent false positive remember to w
ash the plate well.
6. We reccomend d
uplication for both standard and sample testing.
7.
Do not let the plate to dry during assay  as active components will be inactivated.
8. To avoid cross contamination d
o not reuse tips and tubes.
9. Do not use reagents from different batchs t
ogether.



Application example

Detection of GSH levels in plant leaves

GSH ELISA kit tested on plant leafs

Material:
Arabidopsis thaliana leaves.