GA3 | Gibberellic acid

473 €

AS06 194  |  clonality: polyclonal  |  host: rat



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Item No:
AS06 194

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product information

Gibberellic Acid (GA) is a very potent plant hormone, which regulates  the growth rate of plants promoting cell elongation. Gibberellic acid stimulates the cells of germinating seeds to produce mRNA molecules that code for hydrolytic enzymes. It was first discovered in Japan, in 1935 as a result of the study of a condition common in rice plants called "foolish seedling" disease, which caused the plants to grow much taller than normal.


BSA-conjugated gibberellic acid (gibberellin A3)

Host Rat
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7.35
Quantity 100 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water and 50 ĩl of glycerol

store lyophilized/reconstituted at -20°C; this aliquote can be freezed and thawed for up to five times and showed stability for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications immunolocalization (IL)
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AS010 933 | GA3-BSA | gibberellic acid BSA conjugate

collection of antibodies to plant hormones

secondary antibodies to rat IgG

Secondary antibodies

Additional information

Total IgG concentration is 20 mg/ml. Antibody has low affinity to free GA and other GA forms: 1,4,5,7,9,53 as tested by competitive ELISA.

Using a conjugated Gibberellic acid 3-Ethyl-Chloroformiate-(PC), antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio (a) Gibberellic acid 3-ECF-BSA (GA3-BSA) 1 Gibberellic acid 1-ECF-BSA (GA1-BSA) 1/> 50 000 Gibberellic acid 4-ECF-BSA (GA4-BSA) 1/> 50 000 Gibberellic acid 5-ECF-BSA (GA5-BSA) 1/> 50 000 Gibberellic acid 7-ECF-BSA (GA7-BSA) 1/> 50 000 Gibberellic acid 9-ECF-BSA (GA9-BSA) 1/> 50 000 Gibberellic acid 53-ECF-BSA (GA53-BSA) 1/> 50 000 (a) : Gibberellic acid 3-ECF-BSA concentration/Other conjugated acids concentration at half displacement ; ECF = Ethyl-Chloroformiate, BSA : Bovine Serum Albumin.

GA3 antibody specificity testing by ELISA

application information
Recommended dilution

1: 100 (IL)

Expected | apparent MW
Confirmed reactivity Euphorbia pulcherrima
Predicted reactivity

dicots and monocots

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

for detailed immunolocalization protocol check here
total IgG was purified by ammonium sulfate precipitation

Selected references Löfke et al. (2013). Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters during root gravitropism. PNAS January 9.

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