Plant/Algal cell antibodies / Environmental stress / Oxidative stress

product information

Glutathione synthetase (GSH-S) catalyses the last step of glutathione (GSH) synthesis. GSH is synthesized in two ATP-dependent steps: in the first, gamma-glutamylcysteine (gamma-EC) is synthesized from L-glutamate and cysteine, catalyzed by gamma-glutamylcysteine synthetase (g-ECS); in the second step, catalysed by GSH-S, glycine is added to the C-terminal end of gamma-EC. GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development

KLH-conjugated synthetic peptide derived from Zea mays GSH-S sequence Q8W4W2

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunolocalization (IL)

AS06 181 | anti-GR | glutathione reductase

collection of antibodies to plant stress proteins

total IgG concentration is 3.7 µg/µl

application information

1:2 000 with standard ECL (WB)

45 kDa

Nicotiana tabacum, Zea mays

dicots including: Arabidopsis thaliana, Pisum sativum, Solanum lycopersicum, monocots including: Hordeum vulgare, Oryza sativa

no confirmed exceptions from predicted reactivity known in the moment

Immunolocalization has been done for Arabidopsis thaliana and Nicotiana tabacum

Gomez et al. (2004) Intercellular distribution of glutathione synthesis in maize leaves and its response to short term chilling. Plant Physiol. 134: 1662-1671.