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Goat anti-Human IgM (” chain), F(ab)'2 fragment, ALP conjugated
AS10 1237 | Clonality: Polyclonal | Host:Goat | Reactivity: Human IgM (µ chain)
- Product Info
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Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified IgG, F(ab)'2 fragment. Format: Liquid Quantity: 0,5 mg Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Recommended dilution: 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB) - Additional Information
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Additional information: This antibody reacts with:heavy (µ) chains on human IgM based on immunoelectrophoresis.
Minimum cross-reactivity is observed to: non-immunoglobulin serum proteins human IgG.
Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)
- Background
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Background: Goat anti-human IgM (µ chain), ALP (alkaline phosphatase) conjugated is a F(ab)'2 fragment of a secondary antibody which binds to human IgM in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG. - Protocols
- Antibody protocols
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