Goat anti-Biotin, DyLightŪ 650 conjugated

197 €

AS12 2228 | clonality: polyclonal | host: goat | reactivity: Biotin


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Item No:
AS12 2228

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product information

Goat anti-Biotin - DyLight® 650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to biotin in immunological assays.

DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are affinity purified using solid phase biotin.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.


purified biotin, whole molecule

Host Goat
Clonality Polyclonal
Purity Affinity purified biotin
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Tested applications
Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

This antibody reacts with Biotin

This antibody does not react with free BSA or free KLH

application information
Recommended dilution

1:20 - 1:2,000 for most applications

Selected references

Ainla et al. (2013). Lab on a Biomembrane: rapid prototyping and manipulation of 2D fluidic lipid bilayers circuits. Sci Rep. 2013 Sep 25;3:2743. doi: 10.1038/srep02743.

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