Goat anti-Human IgG Fc, F(ab)'2 fragment, HRP conjugated

197 €

AS10 810 | clonality: polyclonal  |  host: goat  | reactivity: human IgG Fc (two heavy chains with constant domains)


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Item No:
AS10 810

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product information

Goat anti-human IgG Fc (two heavy chains with constant domains), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.


Purified human IgG

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Related products

antibodies to human immunoglobulins

Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.

No reactivity is observed with the light chains on human immunoglobulins and non-immunoglobulin human serum proteins and human IgG F(ab)'2 fragment based in immunoelectrophoresis.

application information
Recommended dilution

optimal working dilution should be determined by the investigator

Selected references

to be added when available

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