Goat anti-Human IgG (H&L), F(ab)'2 fragment, TRITC conjugated

197 €

AS10 946 |clonality: polyclonal  | host: goat | reactivity: human IgG (H&L) F(ab)'2 fragment


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Item No:
AS10 946

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product information

Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary, Rhodamine (TRITC-rhodamine - Tetramethylrhodamine-5-isothiocyanate) conjugated antibody, which is reacting against human IgG (H&L), F(ab)'2 fragment. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG, F(ab)'2 fragment
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Related products other secondary antibodies to human immunoglobulins
Tested applications
Additional information

This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

application information
Recommended dilution Needs to be determined by end user for immunocytochemistry (ICC), immunohistochemistry (IHC) and for flow cytometry.
Selected references

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