Goat anti-Human kappa chain, ALP conjugated, min. cross-reactivity to Mouse serum

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AS10 1410 | clonality: polyclonal | host: goat | reactivity: human kappa chain


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Item No:
AS10 1410

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product information
Background Goat anti-human kappa (k) chain is a secondary antibody conjugated to ALP (alkaline phosphatase), which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.
Immunogen Purified human Kappa Chain BAA37169.1
Host Goat
Clonality Polyclonal
Purity Affinity purified IgG
Format Liquid
Quantity 0.5 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Related products other ALP (alkaline phosphatase) conjugated secondary antibodies against human immunoglobulins
Tested applications
Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with: kappa light chains on human immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to:non-immunoglobulin human serum proteins,heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins,mouse serum proteins.

application information
Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:2000 for Immunohistochemistry 1:50 000-1:5 000 for ELISA/Western blot
Selected references

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