Goat anti-Mouse IgG (H&L), HRP conjugated

141 €
AS11 1772 | clonality: polyclonal | host: goat | reactivity: mouse IgG (H&L)


4 st
Item No:
AS11 1772

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product information
Background Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L).
Immunogen Purified mouse IgG (H&L) AAA51107
Host Goat
Clonality Polyclonal
Purity Affinity purified IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Related products other HRP-conjugated secondary antibodies against mouse IgG (H&L)
Tested applications
Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with:heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins.

The antibody will detect all isotypes of mouse IgG. 

application information
Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:5 000 for Immunohistochemistry 1:200-1:5000 for ELISA/Western blot
Selected references Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64.

application example

western blot on plant recombinant proteins using anti-His antibody

500 femtomoles of His-tagged proteins IsiA, NifH, PsbA, PsbB and PetC were loaded per gel well in Agrisera PEB extraction buffer.  Proteins were separated on  4-12 % NuPAGE PAGE Bis-Tris polycacrylamide gel (Invitrogen) and blotted 1h to PVDF. Blots were blocked with   for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat, anti-mouse IgG horse radish peroxidase conjugated, from Agrisera AS11 1772) diluted to 1:25 000 in 2 % ECL Advance blocking reagent for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was  5 seconds.

Apparent molecular weight of recombinant proteins: IsiA - 27 kda, NifH - 34 kDa, PsbA - 30-37 kDa, PsbB - 40 kDa, PetC - 23 kDa.

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