Goat anti-Mouse IgG (H&L), ALP conjugated, min. cross-reactivity to bovine, horse, human, pig or rabbit serum

197 €
AS10 1424 | clonality: polyclonal | host: goat | reactivity: mouse IgG (H&L)


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Item No:
AS10 1424

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product information
Background Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to ALP (alkaline phosphatase), which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase mouse IgG (H&L).
Immunogen Purified mouse IgG (H&L) AAA51107
Host Goat
Clonality Polyclonal
Purity Affinity purified IgG
Format Liquid
Quantity 1 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Related products other secondary antibodies against mouse IgG (H&L), ALP conjugated
Tested applications
Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1% (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with:heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins serum proteins from bovine, horse, human, pig, or rabbit.

application information
Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:2000 for Immunohistochemistry 1:50 000-1:5 000 for ELISA/Western blot
Selected references

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