Goat anti-Rabbit IgG Fc, HRP conjugated

175 €

AS10 858 | clonality: polyclonal  |  host: goat  | reactivity: rabbit IgG Fc (two heavy chains with constant domains)


1 st
Item No:
AS10 858

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product information

Goat anti-rabbit IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to rabbit IgG Fc (two heavy chains with constant domains) in immunological assays.


Purified Rabbit IgG

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Related products

other enzyme conjugated secondary antibodies to rabbit IgG

Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

This antibody reacts with the heavy chains on rabbit IgG based on immunoelectrophoresis.

No reactivity is observed to the light chains on rabbit immunoglobulins or non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

This antibody will cross react with the heavy chain on other species IgG, but not with IgM or IgA.

application information
Recommended dilution

optimal working dilution should be determined by the investigator

Selected references

to be added when available

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