Goat anti-Rabbit IgG (H&L), DyLightŪ 550 conjugated, min. cross-reactivity to bovine,goat,human, mouse,Rat IgG - free sample

AS11 1782-free   | clonality: polyclonal  |  host:goat   |  reactivity:rabbit IgG (H&L)


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Item No:
AS11 1782-free

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product information

Goat anti-rabbit IgG (H&L), DyLight® 550 conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Fluor to protein ratio is 2.47 moles DyLight® 550 per mole antibody. Antibodies are adsorbed against bovine,goat,human,mouse,rat IgG and are affinity purified using solid phase rabbit IgG.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Host Goat
Clonality polyclonal
Purity affinity purified IgG
Format Liquid
Quantity 0.01 mg
Storage Store material at 2-8°C for short term use. 

For long time storage dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Related products other DyLight® 488 conjugated conjugated secondary antibodies to rabbit IgG

Tested applications
Additional information

This antibody reacts with the heavy (gamma) chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,goat,human,mouse,rat IgG based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

application information
Recommended dilution 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC)
Selected references

aplication example
immunolocalization of BiP protein in plant tissue using DyLight550 secondary antibody
BiP localization in 4 days old Arabidopsis thaliana roots.  BiP signal shown in red, Co-staining with DAPI visualized nucleus (blue color). The material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Rabbit anti-BiP primary antibody diluted in 1: 400 and goat anti-rabbit IgG, pre-adsorbed secondary antibody DyLight®550 conjugated (Agrisera) have been used. Scale bar – 10 µm.

Courtesy Dr. Taras Pasternak, Freiburg University

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