Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated, min. cross-reactivity to bovine,human, mouse IgG/serum

197 €

AS10 1146   | clonality: polyclonal  |  host:goat   |  reactivity:rabbit IgG (H&L)


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Item No:
AS10 1146

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product information
Background Goat anti-rabbit IgG (H&L),  HRP conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to rabbit IgGs in immunological assays. Antibodies are adsorbed against bovine,human,mouse IgG/serum and affinity purified. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Host Goat
Clonality Polyclonal
Purity Affinity purified IgG, F(ab)'2 fragment
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Tested applications
Additional information

This antibody reacts with:heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins serum proteins from bovine, human or mouse IgG from human or mouse.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

application information
Recommended dilution 1:500-1: 5000 for immunohistochemistry (IHC), 1:10 000-1:50 000 for ELISA/western blot.
Selected references

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