Goat anti-Chicken IgY (H&L), HRP conjugated
AS09 603 | clonality: polyclonal | host: goat | reactivity: hen IgY (H&L)
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|Recommended dilution||1 : 10 000 -1 : 150 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 20 000 and 1 : 10 000 (WB)|
|Expected | apparent MW|
|Confirmed reactivity||Chicken IgY heavy and light chains (H&L).|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
Antibody binds to:
Chicken immunoglobulin is often called hen or chicken IgG, however it is derived from egg yolk, therefore IgY.
|Selected references||Huang et al. (2015). Effects of exogenous salicylic acid on the physiological characteristics of Dendrobium officinale under chilling stress. Plant Growth Regulation pp 1-10.|
5 µg of total extract from (1) Hordeum vulgare total leaf, (2) Zea mays (3) Spinacia oleracea extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary anti-PsbA antibody (AS01 016) at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-hen IgG horse radish peroxidase conjugated, AGRISERA) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.
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