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Goat anti-Human IgA heavy (alpha chain), F(ab)'2 fragment, ALP conjugated

227 €

AS10 744 | clonality: polyclonal  |  host: goat  | reactivity: human IgA heavy (alpha chain), F(ab)'2 fragment

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AS10 744

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product information
Background

Goat anti-human IgA (alpha chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgA alpha chains, F(ab)'2 fragments in immunological assays.

Immunogen

purified human IgA, alpha chain

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG
Format Liquid
Quantity 0.5 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC)
Related products

other secondary antibodies to human immunoglobulins

Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.

Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.

application information
Recommended dilution

1: 500 - 2 000 (ELISA) and  (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)

Expected | apparent MW
Confirmed reactivity

human IgA heavy (alpha chains), F(ab)'2 fragment

Predicted reactivity

human IgA heavy (alpha chains), F(ab)'2 fragment

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

No reactivity is observed to light chains or non-immunoglobulin human serum proteins based on immunoelectrophoresis.

Selected references

to be added when available



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