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Goat anti-Human IgA heavy (alpha chain), ALP conjugated, min. cross-reactivity to bovine/mouse/rabbit serum

181 €

AS10 738 | clonality: polyclonal  |  host: goat  | reactivity: human IgA heavy (alpha chain)

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AS10 738

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product information
Background

Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgA heavy, alpha chains in immunological assays.

Immunogen

purified human IgA, alpha chain

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG
Format Liquid
Quantity 0.5 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC)
Related products

other secondary antibodies to human immunoglobulins

Additional information

AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

application information
Recommended dilution

1: 500 - 2 000 (ELISA) and  (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)

Expected | apparent MW
Confirmed reactivity human IgA, heavy alpha chains
Predicted reactivity

human IgA, heavy alpha chains

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

No reactivity is observed to light chains or non-IgA human serum proteins based in immunoelectrophoresis

no reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis

Selected references

to be added when available



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