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Goat anti-Human IgE heavy (epsilon chain), HRP conjugated, min. cross-reactivity bovine/mouse/rabbit serum

262 €

AS10 760 | clonality: polyclonal  |  host: goat  | reactivity: human IgE heavy (epsilon chain)

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AS10 760

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product information
Background

Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to HRP which binds to human IgE heavy (epsilon chain) in immunological assays.

Immunogen

purified human IgE

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Related products

antibodies to human immunoglobulins

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

application information
Recommended dilution

optimal working dilution should be determined by the investigator

Expected | apparent MW
Confirmed reactivity

human IgE heavy (epsilon chain)

Predicted reactivity

human IgE heavy (epsilon chain)

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

No reactivity is observed to the light chains or non-immunoglobulin human serum proteins.

No reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.

Selected references

to be added when available



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