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Goat anti-Human IgG Fc, HRP conjugated, min. reactivity to human IgA and IgM

181 €

AS10 664 | clonality: polyclonal  |  host: goat  | reactivity: human IgG Fc

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AS10 664

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product information
Background

Goat anti-human IgG Fc  is a secondary antibody conjugated to HRP which binds to human Fc part of IgG in immunological assays. The antibody is absorbed agains human IgA and IgM

Immunogen

affinity purified human IgG Fc part

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA), western blot (WB), immunohistochemistry (IHC)
Related products

antibodies to human IgG

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative.

application information
Recommended dilution

1: 10 000 -1: 100 000 (ELISA), (WB), 1: 500 -1: 5000 (IHC)

Expected | apparent MW
Confirmed reactivity

human IgG heavy chain Fc based on immunoelectrophoresis

Predicted reactivity

human IgG Fc

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

No reactivity to serum proteins from bovine or rabbit, No reactivity is observed to human IgA or IgM based on immunoelectrophoresis

Selected references

to be added when available



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