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Goat anti-Human IgG (H&L), F(ab)'2 fragment, DyLightŪ 594 conjugated, min. cross-reactivity to bovine, mouse, rabbit serum

181 €

AS12 2020 | clonality: polyclonal  |  host: goat  | reactivity: human IgG

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AS12 2020

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product information
Background

Goat anti-human IgG (H&L), F(ab)'2 fragment , DyLight® 594 Conjugated, min. cross-reactivity to bovine, mouse, rabbit serum is a secondary antibody conjugated to  DyLight® 594, which binds to all human IgG (H&L), F(ab)'2 fragment in immunological assays. 

DyLight® 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase human IgG (H&L).

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Immunogen

Purified human IgG (H&L), F(ab)'2 fragment

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified Goat IgG
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products

other dylight anti-human secondary antibodies

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

application information
Recommended dilution

1:20 - 1:2,000 for most applications

Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information

Based on  immunoelectrophoresis, this antibody reacts with:  heavy (γ) chains on human IgG (γchain)  light chains on all human immunoglobulins.

No reactivity is observed to: non-immunoglobulin human serum proteins serum proteins from bovine, mouse or rabbit.

Selected references

to be added when available



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