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Goat anti-Human IgG (H&L), F(ab)'2 fragment, HRP conjugated

181 €

AS10 779 | clonality: polyclonal  |  host: goat  | reactivity:human IgG (H&L), F(ab)'2 fragment

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AS10 779

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product information
Background

Goat anti-human IgG (H&L), F(ab)'2 fragment  is a secondary antibody conjugated to HRP which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.

Immunogen

purified human IgG (H&L)

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Related products

HRP-conjugated antibodies to human immunoglobulins

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.

application information
Recommended dilution

optimal working dilution should be determined by the investigator

Expected | apparent MW
Confirmed reactivity

human IgG (H&L), F(ab)'2 fragment

Predicted reactivity

human IgG (H&L), F(ab)'2 fragment

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.

Selected references

to be added when available



||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at support@agrisera.com