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Goat anti-Human IgG (H&L), F(ab)'2 fragment, HRP conjugated, min. cross-reactivity bovine/mouse/rabbit serum

203 €

AS10 785 | clonality: polyclonal  |  host: goat  | reactivity: human IgG (H&L), F(ab)'2 fragment

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AS10 785

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product information
Background

Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.

Immunogen

purified human IgG (H&L)

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG
Format Liquid
Quantity 0.5 mg
Reconstitution
Storage Store undiluted liquid at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Related products

antibodies to human immunoglobulins

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

Purity ≥90 % based on SDS-PAGE. May contain small amounts of intact IgG

application information
Recommended dilution

optimal working dilution should be determined by the investigator

Expected | apparent MW
Confirmed reactivity human IgG (H&L), F(ab)'2 fragment
Predicted reactivity

human IgG (H&L), F(ab)'2 fragment

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunglobulins based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.

Selected references

to be added when available



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