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Goat anti-Human IgG+IgA+IgM, ALP conjugated

181 €

AS10 662 | clonality: polyclonal  |  host: goat  | reactivity: human IgG+IgA+IgM

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AS10 662

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product information
Background

Goat anti-human IgG+IgA+IgM is a secondary antibody conjugated to ALP (alkaline phosphatase) which binds to heavy and light chains of human immunoglobulins in immunological assays.

Immunogen

Purified human IgG+IgA+IgM

Host Goat
Clonality Polyclonal
Clone
Purity Purified goat IgG
Format Liquid
Quantity 1 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Related products
Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.

Antibody concentration is 1.5 mg/ml.

application information
Recommended dilution

1: 500 - 2 000 (ELISA) and  (WB), 1: 20 -1: 2000 (IHC)

Expected | apparent MW
Confirmed reactivity

heavy and light chains of human IgG+IgA+IgM

Predicted reactivity

heavy and light chains of human IgG+IgA+IgM

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

No reactivity is observed to non-immunoglobulin human serum proteins based in immunoelectrophoresis. 

Selected references

to be added when available



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