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Goat anti-Human IgM (ĩ chain), F(ab)'2 fragment, ALP conjugated, min. cross-reactivity to human IgG or IgA

238 €

AS10 1298   | clonality: polyclonal  |  host:goat   |  reactivity:human IgM (µ chain)

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AS10 1298

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product information
Background Goat anti-human IgM (µ chain), ALP (alkaline phosphatase) conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to human IgM in immunological assays. Antibodies are adsorbed against human IgG or IgA. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified IgG, F(ab)'2 fragment
Format Liquid
Quantity 0.5 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products other ALP conjugated secondary antibodies to human IgM
Additional information

This antibody reacts with:heavy (µ) chains on human IgM based on immunoelectrophoresis.

Minimum cross-reactivity is observed to: non-immunoglobulin serum proteins human IgG or IgA.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

application information
Recommended dilution 1:500-1:2000 for ELISA/western blot, 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC) and flow cytometry.
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references


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