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Goat anti-Mouse IgG2, FITC conjugated

339 €

AS15 3052   | clonality: polyclonal  |  host: goat   |  reactivity:mouse IgG2

PRODUCT INFORMATION IN PDF

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AS15 3052

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product information
Background Goat anti-mouse IgG2, FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which is subclass specific and binds to mouse IgG1 in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody.
Immunogen

Purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b.

Host Goat
Clonality Polyclonal
Clone
Purity Purified IgG
Format Lyophilized, for reconstitution add 1 ml of sterile water
Quantity 1 ml
Reconstitution
Storage

Stored lyophilize material at +4°C; and for long time storage at -20°C. 

After reconstirution make aliquots and store at -20°C to avoid repeated freeze-thaw cycles. Prior to use, thawed  the aliquotslowly in the dark at ambient temperature, spun down again and prepare the working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2).  Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. 

Tested applications ELISA (ELISA), Immunocytochemistry (ICH), Immunohistochemistry (IHC)(frozen), immunofluorescence (IF)
Related products other FITC conjugated secondary antibodies to mouse IgG
Additional information

If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

The reactivity of this product is directed to the subclasses IgG2a and IgG2b as tested against mouse sera other purified homogenous immunoglobulins.

application information
Recommended dilution

1:20 to 1:80
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Expected | apparent MW
Confirmed reactivity

Mouse IgG2a and IgG2b, subclass specific

Predicted reactivity
Not reactive in It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Additional information
Selected references not available, antibody released in November 2015.


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