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Goat anti-Rabbit IgG (H&L), ALP conjugated

181 €

AS09 607 clonality: polyclonal host: goat reactivity: rabbit IgG (H&L)

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Item No:
AS09 607

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product information
Background

Goat anti-rabbit IgG (H&L)  is a secondary antibody conjugated to AP or ALP (Alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.

Immunogen

Purified Rabbit IgG, whole molecule.

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG.
Format Liquid, clear, colorless.
Quantity 1 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related products

AS09 602 | Goat anti-rabbit IgG (H&L) HRP conjugated

AS09 608 | Goat anti-rabbit IgG (H&L) biotin conjugated

Additional information

Antibody has been affinity purified on solid phase rabbit IgG (H&L).

AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

application information
Recommended dilution

1: 500 - 8 000 (ELISA) and (WB), 1: 500 -1: 2000 (IHC)

Expected | apparent MW
Confirmed reactivity

Rabbit IgG heavy and light chains (H&L).

Predicted reactivity
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Based upon IEP, this antibody binds to:
heavy chains on rabbit IgG
light chains on all rabbit immunoglobulins

No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.

Selected references Krasuska et al. (2015). Switch from heterotrophy to autotrophy of apple cotyledons depends on NO signal. Planta. 2015 Jul 18.


||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at support@agrisera.com