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Goat anti-Rabbit IgG (H&L), DyLightŪ 350 conjugated

181 €

AS11 1812| clonality: polyclonal  |  host: goat  | reactivity: rabbit IgG (H&L)

PRODUCT INFORMATION IN PDF

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AS11 1812

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product information
Background

Goat anti-rabbit IgG, DyLight® 350 Conjugate is a secondary antibody conjugated to
DyLight® 350, which binds to all rabbit IgGs in immunological assays.

DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Immunogen

Purified Rabbit IgG, whole molecule

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF)
Related products

other anti-rabbit DyLight® conjugated secondary antibodies

Plant and algal protein extraction buffer

Additional information

Conjugate is present in 10 mM Sodium Phosphate,
0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.
0.05 % (w/v) sodium azide is added as preservative.

DyLight® 350 has a maximum absorbance at 353 nm; Emax = 432 nm.

application information
Recommended dilution

1:20 – 1:2 000 (IHC), 1:50 – 1:5 000 (ICC), 1: 3000 (IF)

Expected | apparent MW
Confirmed reactivity

rabbit IgG heavy and light chains (H&L)

Predicted reactivity

rabbit IgG heavy and light chains (H&L)

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.

No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis. 

Purity of this antibody is > 95% based on SDS-PAGE.

Selected references

to be added when available



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