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Goat anti-Rabbit IgG (H&L), DyLightŪ 594 conjugated

181 €

AS11 1815| clonality: polyclonal  |  host: goat  | reactivity: rabbit IgG (H&L)

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AS11 1815

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product information
Background

Goat anti-rabbit IgG, DyLight® 594 Conjugate is a secondary antibody conjugated to DyLight® 594, which binds to all rabbit IgGs in immunological assays.

DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Immunogen

Purified Rabbit IgG, whole molecule

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)
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Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

DyLight® 594 (Ex = 593 nm; Em = 618 nm)

application information
Recommended dilution 1 : 50- 1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC), 1 : 3000 (IF)
Expected | apparent MW
Confirmed reactivity Rabbit IgG heavy and light chains (H&L)
Predicted reactivity Rabbit IgG Heavy and Light chains (H&L)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.

No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.

Purity of this antibody is 95% based on SDS-PAGE.

Selected references Chong et al. (2015). Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans. BMC Biotechnol. 2015 Jun 18;15:56. doi: 10.1186/s12896-015-0154-8.


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