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Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, ALP (alkaline phosphatase) conjugated, min. cross-reactivity to bovine,human, mouse IgG/serum

203 €

AS10 1017  | clonality: polyclonal  |  host: goat  |  reactivity:rabbit IgG (H&L)

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AS10 1017

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product information
Background Goat anti-rabbit IgG (H&L) is a  F(ab)'2 fragment of ALP (alkaline phosphatase) conjugated secondary antibody which binds to all rabbit IgGs in immunological assays. Antibody is adsorbed to bovine,human, mouse IgG/serum and its purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG, F(ab)'2 fragment
Format Liquid
Quantity 0.5 mg
Reconstitution
Storage Store undiluted liquid at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products other ALP conjugated secondary antibodies to rabbit IgG
Additional information

This antibody reacts with:heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, goat, human, mouse or rat.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free and , 0.05 % (w/v) sodium azide as preservative.

application information
Recommended dilution 1:500-1:2000 for ELISA/western blot, 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC). (ICC).
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
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