Goat anti-rabbit IgG (H&L) -F(ab)'2 fragment, DyLight®650 Conjugated, min. cross-reactivity to bovine, human, or mouse lgG or serum proteins is a secondary antibody conjugated to DyLight® 650, which binds to Rabbit IgG (H&L) - F(ab)'2 fragment in immunological assays.
DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are purified using solid phase Rabbit IgG
DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Purified Rabbit IgG, whole molecule
Affinity purified goat IgG
For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.