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Goat anti-Rabbit IgG (H&L), HRP conjugated, min. cross-reactivity to bovine/Human/goat/mouse/rat IgG

181 €

AS10 667| clonality: polyclonal  |  host: goat  | reactivity: rabbit IgG (H&L)

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AS10 667

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product information
Background

Goat anti-rabbit IgG  is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.

Immunogen

Purified Rabbit IgG, whole molecule (H&L chains)

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications immunohistochemistry (IHC), ELISA (ELISA), western blot (WB)
Related products

AS09 602 | Goat anti-rabbit IgG (H&L) HRP conjugated

antibodies to rabbit immunoglobulins

Additional information

No reactivity is observed to bovine/goat/human/mouse or rat IgG based on immunoelectrophoresis.

No reactivity to non-immunoglobulin rabbit serum proteins.

Antibody is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % (v/v)Kathon CG is added as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.

DyLight™ 488 has a maximum absorbance at 493 nm; Emax = 518 nm
Fluor to protein ratio is: 1-5 Moles DyLight™ 488 per Mole Antibody

application information
Recommended dilution

1:500 – 1:5 000 (IHC), 1:200 – 1:5 000 (ELISA) (WB)

Expected | apparent MW
Confirmed reactivity

rabbit IgG heavy and light chains (H&L)

Predicted reactivity

rabbit IgG heavy and light chains (H&L)

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis. 

Selected references

to be added when available



||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com