Goat anti-Rabbit IgG (H&L), HRP conjugated, min. cross-reactivity to bovine/Human/goat/mouse/rat IgG

181 €

AS10 667| clonality: polyclonal  |  host: goat  | reactivity: rabbit IgG (H&L)


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Item No:
AS10 667

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product information

Goat anti-rabbit IgG  is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.


Purified Rabbit IgG, whole molecule (H&L chains)

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Related products

AS09 602 | Goat anti-rabbit IgG (H&L) HRP conjugated

antibodies to rabbit immunoglobulins

Tested applications immunohistochemistry (IHC), ELISA (ELISA), western blot (WB)
Additional information

No reactivity is observed to bovine/goat/human/mouse or rat IgG based on immunoelectrophoresis.

No reactivity to non-immunoglobulin rabbit serum proteins.

Antibody is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % (v/v)Kathon CG is added as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.

DyLight™ 488 has a maximum absorbance at 493 nm; Emax = 518 nm
Fluor to protein ratio is: 1-5 Moles DyLight™ 488 per Mole Antibody

No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis. 

application information
Recommended dilution

1:500 – 1:5 000 (IHC), 1:200 – 1:5 000 (ELISA) (WB)

Selected references

to be added when available

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