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Goat anti-Rat IgG (H&L), ALP conjugated, min. reactivity to Human and mouse IgG, highly adsorbed against mouse IgG

216 €

AS10 663 | clonality: polyclonal  |  host: goat | reactivity: rat IgG (H&L)

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AS10 663

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product information
Background

Goat anti-rat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.

Immunogen

Purified Rat IgG, whole molecule

Host Goat
Clonality Polyclonal
Clone
Purity Purified goat IgG
Format Liquid
Quantity 1 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC)
Related products
Additional information

APL conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

application information
Recommended dilution

1: 500 - 2 000 (ELISA) and  (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)

Expected | apparent MW
Confirmed reactivity heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on IEP.
Predicted reactivity

heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on IEP.

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody is highly cross absorbed against mouse IgG. No reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis. No reactivity is observed to human or mouse IgG based on immunoelecrophoresis. 

Selected references

to be added when available



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