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Goat anti-Guinea pig IgG (H&L), HRP conjugated

181 €

AS10 653 | clonality: polyclonal | host: goat | reactivity: guinea pig IgG (H&L)

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AS10 653

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product information
Background

Goat anti-guinea pig IgG is a secondary antibody conjugated to HRP which binds to all guinea pig IgGs in immunological assays.

Immunogen

Purified Guinea pig IgG, whole molecule

Host Goat
Clonality Polyclonal
Clone
Purity Purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Related products

AS09 619 | Goat anti-rat IgG (H&L) biotin conjugated

AS06 620 | Goat anti-rat IgG (H&L) ALP(alkaline phosphatase) conjugated

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative.

application information
Recommended dilution

1: 10 000 -1: 50 000 (ELISA), (WB), 1: 500 -1: 5000 (IHC)

Expected | apparent MW
Confirmed reactivity

guinea pig IgG heavy and light chains (H&L) of all guinea pig immunoglobulins

Predicted reactivity

guinea pig IgG heavy and light chains (H&L) of all guinea pig immunoglobulins

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody reacts with the heavey chaings on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.

Selected references

to be added when available



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