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GST-tag

266 €

AS15 2861   | clonality: monoclonal  |  host: mouse  |  reactivity:GST-tag

PRODUCT INFORMATION IN PDF

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AS15 2861

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product information
Background GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
Immunogen GST protein
Host Mouse
Clonality Monoclonal
Clone IgG2a, clone GST.B6
Purity
Format Lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide
Quantity 50 µg
Reconstitution

For reconstitution add 50 ĩl of sterile water.

Storage

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), dot blot (Dot), ELISA (ELISA), immunoprecipitation (IP), immunohistochemistry (IHC)
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Additional information

Exact working dilution for Dot Blot, ELISA and  IP needs to be determined experimentally.

application information
Recommended dilution

1 : 5000 (0.2 µg/µl) with ECL (WB)

Expected | apparent MW

26 kDa

Confirmed reactivity

native and denatured forms of purified GST or GST fusion proteins

Predicted reactivity native and denatured forms of purified GST or GST fusion proteins
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information Antibody is purified on Protein A
Selected references

to be added when available antibody available in March 2015



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