H3 | histone H3 (chicken polyclonal)

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AS15 2855 | clonality: polyclonal | host: chicken | reactivity: A. thaliana, C. reinhardtii, L. esculentum | cellular [compartment marker] of nucleoplasm


32 st
Item No:
AS15 2855

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product information

Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.


KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At5g10980), H3.2 P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2 Q9FXI7 (At1g19890)

Host Chicken
Clonality Polyclonal
Purity Total IgY
Format Liquid in PBS, pH 8.5 with 0.02 % sodium azide
Quantity 50 ĩl
Storage store at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications western blot (WB)
Related products

AS11 1804 | RNA polymerase II subunit B1, rabbit antibody

AS10 710 | anti-H3 | anti-histone H3, rabbit antibody

AS10 710PRE | H3 | histone H3, pre-immune serum

collection of antibodies to DNA/RNA/cell cycle

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii

application information
Recommended dilution

1 : 2500 (WB)

Expected | apparent MW

15 | 17 kDa

Confirmed reactivity

Arabidopsis thaliana, Chlamydomonas reinhardtii, Lycopersicum esculentum

Predicted reactivity

Arabidopsis thaliana, Brassica napus, Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Hordeum vulgare, Medicago sativa, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Pinus pinaster, Pisum sativum, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Triticum aestivum, Zea mays, Vicia faba, Vitis vinifera, Volvox sp.

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information to be added when available
Selected references to be added when available, antibody release in June 2015.

application example

western blot using anti-plant histone 3, chicken antibody
From left to right: 2 wells of nuclear + cytoplasmic enriched protein, 2 wells of total proteins of Chlamydomonas reinhardtii protein saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (DayLight 800) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.

Courtesy of Dr. Betty Chung, University of Cambridge, United Kingdom

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