H3 | Histone H3 (affinity purified)
AS10 710A | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, S. lycopersicum, V. faba, P. patens, S. europaea, Z. mays, Ch. reinhardtii | cellular [compartment marker] of nucleoplasm
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|Recommended dilution||2.5 ĩg/100 ĩg of chromatin (ChIp-qPCR), 1 : 5000 (WB)|
|Expected | apparent MW||
15 | 17 kDa
|Confirmed reactivity||Arabidopsis thaliana|
Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Chlamydomonas reinhardtii, Physcomitrella patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays Brachypodium distachyon, Brassica napus, Hordeum vulgare, Nicotiana tabacum, Malus domestica, Medicago sativa, Triticum aestivum, Pinus pinaster, Pisum sativum, Oryza sativa, Zea mays, Vitis vinifera, Volvox sp.
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
Protocol for isolation of cytosolic and nuclear fractions can be found here.
Specific protol for ChIP can be found here: Saleh et al. (2008).
|Selected references||To be added when available, antibody released in April 2016.|
Chromatin Immunoprecipitation: using anti-plant Histone 3 polyclonal antibodies. Chromatin from Arabidopsis thaliana wilde type, deacetylase mutant and over-expressors was cross-linked using formaldehyde. Chromatin was isolated and DNA was sheared along with the bound protein by sonictaion. DNA-protein complex was immunoprecipitated using affinity purified, polyclonal anti-Histone 3 antibodies. Immunoprecipitated DNA was quantified using quantitative PCR and normalized to the input chromatin.
Procedure was according to a protocol described here: Saleh et al. (2008).
Courtesy of Dr. Cristián Holzmann, Catholic University of Chile, Chile
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