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H3 | Histone H3 (40 ĩg, HRP-conjugated)

406 €

AS10 710-HRP | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, S. lycopersicum, V. faba, P. patens, S. europaea, Z. mays, Ch. reinhardtii | cellular [compartment marker] of nucleoplasm

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AS10 710-HRP

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product information
Background

Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

Immunogen

KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At5g10980), H3.2 P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2 Q9FXI7 (At1g19890)

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Liquid in PBS pH 7.4, conjugated to HRP
Quantity 40 µg
Reconstitution
Storage

Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Tested applications western blot (WB)
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AS10 710PRE | H3 | histone H3, pre-immune serum

Collection of antibodies to epigenetics/DNA methylation

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii

application information
Recommended dilution

1 : 5000 with standard ECL (WB)

Expected | apparent MW

15 | 17 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Chlamydomonas reinhardtii, Physcomitrella patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays Brachypodium distachyon, Brassica napus, Hordeum vulgare, Nicotiana tabacum, Malus domestica, Medicago sativa, Triticum aestivum, Pinus pinaster, Pisum sativum, Oryza sativa, Zea mays, Vitis vinifera, Volvox sp.

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references To be added when available, antibody released in April 2016.

 

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