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HDA6 | Histone deacetylase 6

195 €

AS14 2787 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


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Item No:
AS14 2787

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product information
Background

HDA6 (histone deacetylase 6) is an enzyme (EC=3.5.1.98) responsible for deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Involved in transcriptional regulation, cell cycle progression and developmental events. Not detected in leaves, stems, flowers and young siliques. Induced by jasmonic acid and ethylene.

Immunogen

KLH-conjugated synthetic peptide chosen from HDA6 protein of Arabidopsis thaliana Uniprot: Q9FML2, TAIR: At5g63110

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

antibodies involved in regulation of transcription

antibodies to environmental stress

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

52 kDa

Confirmed reactivity

Recombinant HDA6 of Arabidopsis thaliana

Predicted reactivity Brassica rapa, Capsella rubella, Citrus clementina, Chlamydomonas reinhardii, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Phaseolus vulgaris, Populus trichocarpa, Physcomitrella patens, Ricinus communis, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Zea mays, Vitis vinifera
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody is so far recognizing recombinant HDA6 overexpressed with GST or MBP in western blot and its reactivity in endogenous extract remains to be optimized. 

Selected references to be added when available, antibody released in February 2016.


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