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HDT3 | Histone deacetylase HDT3

265 €
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AS16 3968 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


PRODUCT INFORMATION IN PDF

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AS16 3968

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product information
Background

HDT3 is probably mediating in the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. This protein is also involved in the modulation of abscisic acid and stress-responsive genes.Synonymes:HD-tuins protein 3, Histone deacetylase 2c

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana HDT3, UniProt: Q9LZR5, TAIR: At5g03740

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products Epigenetics/DNA methylation

Hormones
Additional information
application information
Recommended dilution 1 : 4000-1 : 8000 (WB)
Expected | apparent MW

31.8 | 40 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Noccaea caerulescens
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

to be added when available, antibody released in October 2017


application example

 western blot using anti-HDT3 antibodies
Nuclei were isolated from Arabidopsis thaliana leaves and resuspended with SDS loading buffer and then denatured at 95oC for 10 min. Proteins were separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry or tank transfer. Blots were blocked with 3% milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at 1:4 000 dilution for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBST at RT with agitation. Blot was incubated in radish peroxidase conjugated goat anti-rabbit IgG (AS09 602, Agrisera) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 1 min with Clarity™ Western ECL Substrate (170-5609, BIO-RAD). Exposure time was 100 seconds.
Note: increasing blocking to 10% milk may reduce background signal.

Courtesy of Dr. Xiangsong Cheng, Wisconsin Institute for Discovery, USA

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