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Hirudin | Refludan

304 €

IMS01-134-330  |  clonality: polyclonal  |  host: hen  |  reactivity: human

PRODUCT INFORMATION IN PDF

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Item No:
IMS01-134-330

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product information
Background

Hirudin (P01050) is a highly effective and very specific anticoagulant which specifically inhibits formation of  fibrin and activation of factors V, VIII and XIII, by forming irreversible, noncovalent 1:1 complexes with alpha-thrombin. Hirudin is a 65 amino acid polypeptide, 7 kDa protein, naturally originating from the medical leech Hirudo medicinalis. It is a very potent thrombin inhibitor. Antibody binding to hirudin inhibits the interaction with alpha-thrombin.

Immunogen

purified full length recombinant protein (Refludan®, Hoechst Marion Roussel Inc.)

Host Hen
Clonality Polyclonal
Clone
Purity Total IgY fraction
Format Liquid in 0.9% NaCl, 0.1% sodium azide
Quantity 100 ĩl (18 mg/ml)
Reconstitution
Storage

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB), ELISA (ELISA)
Related products

Secondary antibodies

Additional information

the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation

application information
Recommended dilution

1: 1000 with standard ECL (WB), 1: 1000 (ELISA)

Expected | apparent MW

7 kDa

Confirmed reactivity

hirudin

Predicted reactivity

Hirudo medicinalis

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

reactiv against purified hirudine; no reactivity against normal human plasma in ELISA

Selected references

to be added when available



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