Plant/Algal cell antibodies / Environmental stress / Heat shock

product information

Hsp17.7 belongs to a family of class II of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.

full length recombinant protein produced in E. coli and purified by conventional methods (no affinity tag). Arabidopsis thaliana Hsp17.7 CII (class two) O65376

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS07 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 rabbit antibody

AS08 284 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 hen antibody

collection of antibodies to plant heat shock proteins

to be added when available

application information

1: 1000 with standard ECL (WB)

17.7 kDa

Arabidopsis thaliana, Agave tequiliana var. Weber, Cucumis sativus, Silene vulgaris

higher plants

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.

Luján et al. (2009). Small heat-shock proteins and leaf cooling capacity account for the unusual heat tolerance of the central spike leaves in Agave tequilana var. Weber. Plant Cell Environ. 32(12):1791-803.

application example

15 µg of total protein from (HS) heat shocked (38ºC/2h) Arabidopsis thaliana , (C) Arabidopsis thaliana control plants, (1,2,5,10) 1,2,5,10 ng of recombinant puridfied HSP17.7 were separated on  15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad).  Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000 from Abcam). Development was done ECL (Thermo Scientific) according to the recommendations of the manufacturers.
Image was acquired with a 10 sec exposure time on X-ray film (Kodak). Band at ~38 kDa represents and SDS resistant dimer.