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Plant/Algal cell antibodies
- Protein standards-quantitation
- Global antibodies
- Compartment markers
- Bioenergetics
- Carbohydrates
- Developmental biology
- DNA/RNA/cell cycle
- Environmental stress
- Fermentation
- Food proteins
- Hormones
- Mitochondria | Respiration
- Membrane transport system
- Nitrogen metabolism
- Photosynthesis
- Plant pathogens
- Toxins
- Tag antibodies
- Secondary antibodies/blocking
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Animal cell antibodies
- Bacterial, insect and fungal
- Carrier proteins
- Fish proteins
- Human proteins
- Immunoglobulins
- Neurosteroids/Neurobiology
- Secondary antibodies/blocking
- Secondary antibodies
HSP70 | heat shock protein 70, chloroplastic
AS08 348 | clonality:polyclonal | host:rabbit | reactivity: A. thaliana, O. sativa, P. sativm, P. strobus
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| application information |
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| recommended dilution | 1: 2000 (WB), 1: 100 (IP) |
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| expected | apparent MW | 76 | 70 kDa |
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| confirmed reactivity | Arabidopsis thaliana, Oryza sativa, Pinus strobus, Pisum sativum |
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| predicted reactivity | dicots including: Spinacia oleracea, Vitis vinifera, monocots including: Oryza sativa, Zea mays, trees: Picea sitchemsis, moss: Physomitrella patens |
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| not reactive in | no confirmed exceptions from predicted reactivity known in the moment |
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| additional information | to be added when available |
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| selected references | Yu et al. (2011). Comparative proteomic study reveals the involvement of diurnal cycle in cell division, enlargement and starch accumulation in developing endosperm of Oryza sativa. J of Proteome Res. Nov, ahead of print Pai-Hsiang Su and Hsou-min Li (2011). Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts. Plant Cell. 22(5):1516-31. |
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application example
total protein from Arabidopsis thaliana chloroplasts (20 µg) and ,Arabidopsis thaliana leaf extracts (25 µg) were separated on 10% acrilamide gel and electrophoresis prepared according to Schägger and von Jagov (Anl. Biochem., 1987, 166:368-379). After running the gel, proteins were transferred to nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Transfer was checked by Ponceau S staining. Blot was destained by several quick washings in distilled water and 1 washing in 1X TBS (10 mM T pH 7.5, 150 mM NaCl) (10-15 min.).Blot was blocked by 1.5 hour in 5% milk in TBST (1X TBS, 0,1 20) After blocking blot was washed quickly twice in TBST and incubated 2 hours with primary antibody (dilution 1: 2000 TBST (dilution 1:1000). Washing: two quick washings in TBST and 3 x 10 min. washings in TBST. Then blot was incubated 45-60 min. with a secondary anti-rabbit antibodies conjugated to peroxidase (Sigma, dilution 1:10000) in TBST. Washing: as above. After washing blot was incubated 1-2 min. in ECL solution and exposed to Kodakautoradiography film. Exposure time was 10 seconds. Courtesy Dr. J. Piechota
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||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com

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