HSP70 | heat shock protein 70, chloroplastic

325 €

AS08 348 | clonality:polyclonal | host:rabbit | reactivity: A. thaliana, O. sativa, P. sativm, P. strobus

PRODUCT INFORMATION IN PDF

product information

background

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.

immunogen

KLH-conjugated synthetic peptide derived from higher plant chloroplastic HSP70, inluding Arabidopsis thaliana cpHSC70-1, At4g24280 and cpHSC70-2, At5g49910

antibody format

rabbit

polyclonal

serum,

lyophilized

quantity

200 µl

for reconstitution add 200 µl of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB), immunoprecipitation (IP)

related products

AS08 371 | anti-HSP70 | heat shock protein 70 cytoplasmic

AS08 347 | anti-HSP70 | heat shock protein 70, mitochondrial

collection of antibodies to plant heat shock proteins

additional information

to be added when available

application information
recommended dilution		1: 2000 (WB), 1: 100 (IP)
expected \| apparent MW		76 \| 70 kDa
confirmed reactivity		Arabidopsis thaliana, Oryza sativa, Pinus strobus, Pisum sativum
predicted reactivity		dicots including: Spinacia oleracea, Vitis vinifera, monocots including: Oryza sativa, Zea mays, trees: Picea sitchemsis, moss: Physomitrella patens
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		to be added when available
selected references		Yu et al. (2011). Comparative proteomic study reveals the involvement of diurnal cycle in cell division, enlargement and starch accumulation in developing endosperm of Oryza sativa. J of Proteome Res. Nov, ahead of print Pai-Hsiang Su and Hsou-min Li (2011). Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts. Plant Cell. 22(5):1516-31.

application example

total protein from Arabidopsis thaliana chloroplasts (20 µg) and ,Arabidopsis thaliana leaf extracts (25 µg) were separated on 10% acrilamide gel and electrophoresis prepared according to Schägger and von Jagov (Anl. Biochem., 1987, 166:368-379). After running the gel, proteins were transferred to nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Transfer was checked by Ponceau S staining. Blot was destained by several quick washings in distilled water and 1 washing in 1X TBS (10 mM T pH 7.5, 150 mM NaCl) (10-15 min.).Blot was blocked by 1.5 hour in 5% milk in TBST (1X TBS, 0,1 20) After blocking blot was washed quickly twice in TBST and incubated 2 hours with primary antibody (dilution 1: 2000 TBST (dilution 1:1000). Washing: two quick washings in TBST and 3 x 10 min. washings in TBST. Then blot was incubated 45-60 min. with a secondary anti-rabbit antibodies conjugated to peroxidase (Sigma, dilution 1:10000) in TBST. Washing: as above. After washing blot was incubated 1-2 min. in ECL solution and exposed to Kodakautoradiography film. Exposure time was 10 seconds.

Courtesy Dr. J. Piechota

western blot detection using anti-HSP70 chlroplastic antibodies

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