Plant/Algal cell biology / Environmental stress / Heat shock

product information

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.

KLH-conjugated synthetic peptide derived from higher plant chloroplastic HSP70, inluding Arabidopsis thaliana cpHSC70-1 and cpHSC70-2

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunoprecipitation (IP)

AS08 371 | anti-HSP70 | heat shock protein 70 cytoplasmic

AS08 347 | anti-HSP70 | heat shock protein 70, mitochondrial

collection of antibodies to plant heat shock proteins

to be added when available

application information

1: 2000 (WB), 1: 100 (IP)

76 | 70 kDa

Arabidopsis thaliana, Pisum sativum

dicots including: Spinacia oleracea, Vitis vinifera, monocots including: Oryza sativa, Zea mays, trees: Picea sitchemsis, moss: Physomitrella patens

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

antibody available in May 2009

application example

total protein from Arabidopsis thaliana chloroplasts (20 µg)  and ,Arabidopsis thaliana leaf extracts (25 µg) were separated on 10% acrilamide gel and electrophoresis prepared according to Schägger and von Jagov (Anl. Biochem., 1987, 166:368-379). After running the gel, proteins were transferred to nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Transfer was checked by Ponceau S staining. Blot was destained by several quick washings in distilled water and 1 washing in 1X TBS (10 mM T pH 7.5, 150 mM NaCl) (10-15 min.).Blot was blocked by 1.5 hour in 5% milk in TBST (1X TBS, 0,1 20) After blocking blot was washed quickly twice in TBST and incubated 2 hours with primary antibody (dilution 1: 2000 TBST (dilution 1:1000). Washing: two quick washings in TBST and 3 x 10 min. washings in TBST. Then blot was incubated 45-60 min. with a secondary anti-rabbit antibodies conjugated to peroxidase (Sigma, dilution 1:10000) in TBST. Washing: as above. After washing blot was incubated 1-2 min. in ECL solution and exposed to Kodakautoradiography film. Exposure time was 10 seconds.

Courtesy Dr. J. Piechota