HSP70 | Heat shock protein 70, mitochondrial

265 €

AS08 347 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


20 st
Item No:
AS08 347

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product information

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.


KLH-conjugated peptide conserved in higher plant mitochondrial HSC70 including Arabidopsis thaliana mtHSC70-1 Q8GUM2, At4g37910 and mtHSC70-2 Q9LDZ0, At5g09590

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized
Quantity 2 x 50 µg
Reconstitution For reconstitution add 50 ĩl of sterile water per tube

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

AS08 371 | anti-HSP70 | heat shock protein 70 cytoplasmic

AS08 348 | anti-HSP70 | heat shock protein 70, chloroplastic

collection of antibodies to heat shock proteins

Additional information
application information
Recommended dilution

1: 1000 (WB)

Expected | apparent MW

73 | 70 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

dicots including: Medicago truncatula, Spinacia oleracea, Phaseolus vulgaris, Pisum sativum, Solanum tuberosum, Vitis vinifera, monoctos including: Oryza sativa, Triticum aestivum, Zea mays, trees: Populus trichocarpa

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.

application example

25 μg of Arabidopsis thaliana leaf extract and 15 μg)Arabidopsis thaliana mitochondrial fraction were separated on 10% gel and blotted on nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Filters where blocked (1.5h) in 5% milk in TBST (1X TBS, 0,1% Tween 20), incubated with 1: 1000 anti-HSP70 mitochondrial antibodies (2h in TBST) followed by incubation with 1: 10 000 secondary anti-rabbit (1h) HRP-coupled antibodies and visualized with standard ECL on Kodak autoradiography film for 15-60 s. Mitochondria were isolated as described by Urantowka et al. (Plant Mol Biol, 2005, 59:239-52). Mitochondrial pellets were suspended in 1X Laemmli buffer (5% beta-mercaptoetanol, 3.7% glycerol, 1.1% SDS, 23 mM Tris- HCl pH 6.8, 0.01% bromophenol blue), heated (95°C, 5 min.) and centrifuged (13 000rpm, 1 min.). Leaf extracts were prepared as described by Martinez-Garcia et al. (Plant J., 1999, 20:251-7).

Courtesy Dr. Janusz Piechota, Wrocław University, Poland



western blot using anti-HSP70 mitochondrial antibodies

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