ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

HSP70 | Salmonid heat shock protein 70, Affinity purified

371 €

AS05 061A  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: salmon

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
20 st
Delivery:
Shipping:
Item No:
AS05 061A

Info: Product suggestions Add review
product information
Background

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

Immunogen

KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 B5X4Z3.

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 200 µg
Reconstitution
For reconstitution add 200 µl of sterile water
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications
Immunohistochemistry (IHC), Immunoprecipiation (IP), Western Blot (WB)
Related products

Collection of antibodies to fish related targets

Secondary antibodies

Additional information

Immunohistochemistry experiments have been done on salmon tissue treated with hydrated autoclaving of formalin fixed sections (unpublished results)

application information
Recommended dilution

1:15 000 with standard ECL (WB), 5 µg of antibodies in reaction mixture (IP), 1: 100 (IHC)

Expected | apparent MW

70 kDa

Confirmed reactivity rainbow trout (Oncorhynchus mykiss)
Predicted reactivity

atlantic salmon (Salmo salar), brook trout (Salvelinus fontinalis)

Not reactive in

No confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody is recognizing the inducible Hsp70 in salmon but not the constitutive.

Selected references Gallant el al. (2017). Physiological responses to a short-term, environmentally realistic, acute heat stress in Atlantic salmon, Salmo salar. FACETS.
Lewis et al. (2016). Different Relationship between hsp70 mRNA and hsp70 Levels in the Heat Shock Response of Two Salmonids with Dissimilar Temperature Preference. Front Physiol. 2016 Nov 7;7:511. eCollection 2016.
Curie et al. (2008). β‐Adrenergic Stimulation Enhances the Heat‐Shock Response in Fish. Physiol & Bioch. Zoology 4:414-425.


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com