HSP90-1 | heat shock protein 90-1

325 €

AS08 346 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Zea mays

product information

background

HSP90-1 (heast shock protein 90-1) is an isoform involved in response to bacterium, arsenic and heat. Synonymes: ATHS83; ATHSP90.1; F6N7.13; F6N7_13; HEAT SHOCK PROTEIN 81-1; HEAT SHOCK PROTEIN 83; HEAT SHOCK PROTEIN 90.1; HSP81-1; HSP81.1; HSP83.

immunogen

full length recombinant Hsp90-1 from Arabidopsis thaliana AT5G52640

antibody format

rabbit;

polyclonal;

serum;

lyophilized

quantity

100 µl

- for reconstitution add 100 µl of sterile water

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

Western blot (WB)

related products

collection of antibodies to plant HSP proteins

additional information

antibody is recognizing both, heat inducible Hsp90-1 and constitutive isofrom Hsp90-2. Both proteins have ca. 85 % similarity.

application information
recommended dilution		1: 3000 with standard ECL (WB)
expected \| apparent MW		80.6 \| 95 kDa (Arabidopsis thaliana)
confirmed reactivity		Arabidopsis thaliana, Zea mays
predicted reactivity		dicots including: Glycine max, Nicotiana benthamina, Nicotiana tabacum, Ricinus communis, Solanum tuberosum, Sorghum bicolor, Vitis vinifera, monocots including: Hordeum vulgare, Oryza sativa, Triticum aestivum, Zea mays, trees: Populus balsamifera, moss: Physcomitrella patens, algae: Micromonas pulsilla, Ostreococcus lucimarinus
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		to be added when available
selected references		Finka et al. (2012). Plasma Membrane Cyclic Nucleotide Gated Calcium Channels Control Land Plant Thermal Sensing and Acquired Thermotolerance. Plant Cell, June 2012. Holding (2011). Pyrophosphate dependent fructose-6-phosphate 1-phosphotransferase induction and attenuation of Hsp gene expression during endosperm modification in Quality Protein Maize. Plant Physiol Dec. 8 (ahead of print).

application example

western blot detection using plant anti-HSP90 antibodies

10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings
that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior
to protein extraction (2). As positive control 20 ng of recombinant Hsp90-2 (3) or Hsp90-1 (4) were separated side by side with the plant samples on 10-15 % gradient SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 3000 for 2h at room temperature with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxides conjugated, from Agrisera, AS09 602, diluted to 1:35 000 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturer's instructions. Exposure time was 30 seconds.

Low salt buffer components are 10 mM Tris (pH 7.6), 68 mM NaCl and 0.05 % Triton X-100.

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